I-Blue Phycocyanin
Ihlathululo
I-Phycocyanin (C-PC) yi-phycobiliprotein encibilika emanzini, eluhlaza okwesibhakabhaka evela khulu kuma-cyanobacteria afana ne-Spirulina platensis kanye ne-Arthrospira maxima. Isebenza njenge-pigment evuna ukukhanya ekukhiqizeni i-photosynthesis ngesikhathi itjengisa izakhiwo ezinamandla zokulwisana nama-oxidant, ukulwa nokuvuvuka, kanye ne-immunomodulatory.
Amakhemikhali nezakhiwo:
Ubunzima bamamolekhyuli: ~240 kDa (ifomu ye-hexameric)
I-Absorption Peak: 620 nm (umbala ohlaza okwesibhakabhaka)
Ukukhanya: Kukhipha ku-650 nm (i-quantum yield ~0.7)
Izakhiwo ze-Spectroscopic
| Ipharamitha | Ukusiza | Ukuqakatheka |
| Isilinganiso sokutjhabalala (ε₆₂₀) | 1.54×106 M−1cm−1 | Izinga lokulinganisa |
| Isikhathi sokuphila sokukhanya | 2.8 ns | Ukuzinza kwe-probe |
| Isivuno se-quantum | 0.68±0.03 | Iinsetjenziswa zokuthatha iinthombe |
| Ukuzinza kwelanga | t1⁄2 >8h (ukukhanya okubonakalako) | Ukucabangela ukwakheka |
Ukuncibilika:
Amanzi: Ancibilika khulu (>50 mg/mL)
I-ethanol: Incibilika kancani (5-10 mg/mL)
Ayincibiliki eentweni eziphilako (isib., i-chloroform, i-hexane)
Ukukhipha nokuHlanjululwa:
Ukukhipha ngamanzi abandako: Kulondoloza ukusebenza kwezinto eziphilako (ukukhiqiza: 10-15% kusuka ku-biomass eyomileko).
I-CO2 eqakathekileko: Ikhulisa ukuhlanzeka (>95%) nge-17 MPa, 45°C.
I-Chromatography: Ukudonswa kwebhedi okungezelelweko (EBA) kwe-C-PC ehlanzekileko ephezulu
Iindlela zokuhlaziya
Ukuhlanzeka: UV-Vis spectrophotometry
Ubunikazi: IKHASI lomdabu + ukufakwa kwe-zinc
Iinsilaphazi: LC-MS/MS (ama-phycotoxins Umsebenzi: Ukuhlolwa kwamandla wokudonswa kwe-oksijini
Iimpharamitha zekhwalithi
| Ibanga | Ubumsulwa (A₆₂₀/A₂₈₀) | Iimbawo |
| Ukudla | >0.7 | Umbala wemvelo |
| Iinthombe | >2.0 | Ukwakheka kwesihloko |
| I-Nutraceutical | >3.0 | Iinthako zokudla |
| Irhubhululo | >4.0 | Iimhloliso ze-biochemical |
Amarhubhelo
✔ I-antioxidant enamandla – Iqeda ama-free radicals
✔ Ukulwisana nokuvuvuka – Kwehlisa ukuvuvuka okungapheliko
✔ Ukusekela amasotja womzimba – Kukhuphula ukusebenza kwama-lymphocyte
✔ Ukuvikelwa kwemizwa – Kusekela ipilo yengqondo
✔ Umbala wemvelo – idayi yokudla eluhlaza okwesibhakabhaka evunywe yi-FDA
Iimbawo
Iinsetjenziswa zokudla: Amakhemikhali, amapuyere (100-500 mg/ngelanga)
Ukudla okusebenzako: I-ayisikhrimu, iinselo (umbala ohlaza satjani wemvelo)
Iinthombe: Amaseramu wokulwisana nokuguga, amakhrimu wokukhanya
Irhubhululo lezepilo: Ama-probe we-fluorescent, ukulethwa kwemithi
Ukusetjenziswa komkhiqizo
